Figure 8. Effect of PL on the expression of apotosis regulatory proteins and on the DNA binding activity of NF-κB.

(a) Expression of DR was determined by Western blotting with antibodies against Fas, DR4 and β-actin (internal control). (b) Expression of apoptosis regulatory proteins was determined by Western blotting with antibodies against cleaved capase-3, cleaved caspase-8, Bax, Bcl-2 and β-actin (internal control). (c) Lung tumors were lysed with A buffer and C buffer. Nuclear extracts were incubated in binding reactions of ³²p-end-labeled oligo nucleotide containing the NF-κB sequence. The present EMSA results are representative for three experiments. (d) Cytosol proteins were used to determine the expression of IκBα, p-IκBα and β-actin (internal control). Nuclear proteins were used to determine the expression of p50, p65 and Histone H1 (internal control) in lung tumor tissues. For the cropped images, samples were run in the same gels under same experimental conditions and processed in parallel. Each band is representative for three experiments. Full-length gels are presented in Supplementary Fig. 8 and Supplementary Fig. 9.