Figure 1. Autophagic process occurs in the entire developmental cycle of Beauveria bassiana.

A hybrid gene of eGFP-BbATG8 was integrated in the wild type strain to track the autophagic process in fungal cells at different development stages. Vacuolar membranes were stained by fluorochrome FM4-64. Punctate and globular signals were observed in germilings, mycelia and spores of both surface (A) and submerged culture (B). In host hemolymph (C), the eGFP signals were also clearly observed in hyphal bodies (in vivo blastospores). Scale bars, 5 μm.