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. 2016 May 20;6:26376. doi: 10.1038/srep26376

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(A) Conidial yield of the wild type, ΔBbCP15 and complementation strains. SDAY plates were used to culture fungal strains, and conidial production was examined at 7 days post inoculation. An asterisk indicates a significant difference in conidial yield between the ΔBbCP15 mutant and wild-type or complementation strains. (B) Western blot analysis indicated that the conidial level of BbCP15p was significantly repressed in ΔBbATG8 mutant, and no obvious protein was detected in disruption mutants without BbATG1 gene. Lane 1: wild type strain; lane 2: ΔBbATG8 mutant; lane 3: ΔBbATG8::BbATG8; lane 4: ΔBbATG1; lane 5: ΔBbATG1::BbATG1; lane 6: ΔBbATG1ΔBbATG8 and lane M: protein marker. SDS-PAGE profile of conidial proteins (30 μg) was used to show the protein integrity.