Table 1. The sequences of primers and probes used in the real-time PCR assay for R. equi detection and for the internal control.

Primer	Target gene	Sequence (5′–3′)	Amplicon size
RhodF	choE	TCGCTCGACAAGACCTAC	120 bp
RhodR		TTCCATCGTCACGCTGT
RhodP		FAM-AGCTGACGATCACGACTCTGCA-BHQ1
hB2M-F	equine β2-M	GACCTGTCTTTCAGCAAGGA	113 bp
hB2M-R		GGGTCTTTGAGAGTAGAGTG
hB2M-P		ROX-TGGTGTGGATGAGTATAGTTGCCG-BHQ2
sB2M-F	swine β2-M	GATCCTTAACCCACTGAGC	122 bp
sB2M-R		AGAGTCAACTCAAAGTAGGTTTGTA
sB2M-P		ROX- GTTCCTAGTCGGATTCATTAACCACTGC-BHQ2