Table 1. Bacterial strains used in this study and comparison of the two conventional PCR methods.

Species		Strains	PCR results
			16S rRNA	BaSS	Realtime PCR
Brucella species
	B. abortus bv. 1 (544)	ATCC 23448	+	+	+
	B. abortus bv. 2 (86/8/59)	ATCC 23449	+	+	+
	B. abortus bv. 3 (Tulya)	ATCC 23450	+	±a)	+
	B. abortus bv. 4 (292)	ATCC 23451	+	+	+
	B. abortus bv. 5 (B3196)	ATCC 23452	+	±	+
	B. abortus bv. 6 (870)	ATCC 23453	+	±	+
	B. abortus bv. 9 (C68)	ATCC 23455	+	±	+
	B. canis (RM6/66)	ATCC 23365	+	±	–
	B. suis bv. 1 (1330)	ATCC 23444	+	±	–
	B. suis bv. 2 (Thomsen)	ATCC 23445	+	±	–
	B. suis bv. 3 (686)	ATCC 23446	+	±	–
	B. suis bv. 4 (40)	ATCC 23447	+	±	–
	B. suis bv. 5 (513)	NCTC 11996	+	±	–
	B. ovis (63/290)	ATCC 25840	+	±	–
	B. neotomae (5K33)	ATCC 23459	+	±	–
	B. melitensis bv. 1 (16M)	ATCC 23456	+	±	–
	B. melitensis bv. 2 (63/9)	ATCC 23457	+	±	–
	B. melitensis bv. 3 (Ether)	ATCC 23458	+	±	–
	B. ceti (B1/94)	NCTC 12891	+	±	–
	B. pinnipedialis (B2/94)	NCTC 12890	+	±	–
	B. microti (CCM 4915)	BCCN 07-01	+	±	–
	B. inopinata (B01)	BCCN 09-01	+	±	–
	Mongolian B. abortus	16 isolates	+	±	+
	Mongolian B. melitensis	94 isolates	+	±	–
	Korean B. abortus	84 isolates	+	+	+
	Korean B. canis	72 isolates	+	±	–
Non-Brucella organisms
	Ochrabactrum anthropi	Field strain	+	–	–
	Escherichia coli O157:H7	Field strain	–	–	–
	Pasteurella multocida	ATCC 43017	–	–	–
	Salmonella Typhimurium	ATCC 14028	–	–	–
	Campylobacter jejuni	ATCC 33560	–	–	–
	Yersinia enterocolitica O:9	NCTC 11174	–	–	–
	Staphylococcus aureus	Field strain	+	–	–
	Clostridium perfringens type A	ATCC 13124	–	–	–

±^a): They were identified with Brucella spp. showing two amplified products (180 bp and 800 bp).