Table 2. Direct detection from clinical specimens of brucellosis-positive Korean native cattle by real-time PCR.

Sample No.	Specimen	Isolationa)	RBTb)	Real-time PCR
				Mean Ct	Tm
1	Buffy coat	+	+	16.89	69.43
2	Buffy coat	+	+	16.88	69.41
3	Calf submandibular LNc)	+	NTd)	29.24	69.23
4	Submandibular LN-1	+	+	28.45	69.46
5	Submandibular LN-2	+	+	29.28	69.15
6	Submandibular LN-3	+	+	29.74	69.42
7	Submandibular LN-4	+	+	28.79	69.38
8	Submandibular LN-5	+	+	29.13	69.08
9	Supramammary LN	+	+	28.89	69.41
10	Calf inguinal LN	+	NT	29.29	69.06
11	Parotid LN	+	-	29.27	69.13
12	Testicle	+	+	28.65	69.34

a) Isolation: The bacterial strains were identified by classical phenotyping and differential multiplex PCR (Kang et al., 2011). b) RBT: Rose-Bengal test. c) LN: Lymph node. d) NT: Not tested.