Table 3. Primers and probes for detection of B. abortus using specific SNPs.
Sequence (5′- 3′) | Comment | ||
---|---|---|---|
Primer | Forward | GATGCGCCGGTTATCCTG | 176 bp size |
Reverse | GTGAAGCCCGCCTGGATG | ||
Probe | Anchor | ACGGAGCATGATGTCATTGGCATAGGAACG | SNP sitea) |
Sensor | ATAGATTTCCGC*CACGGCATCCATC |
a) Single nucleotide polymorphism.