Table 1. Primers used in real-time PCR.
Gene | Sequence of nucleotide (5′–3′) | Size (bp) | Annealing temperature (°C) |
Accession no. | |
---|---|---|---|---|---|
PGR | Fa) | TAATCTGTGGGGATGAAGCA | 181 | 58 | NM 001205356 |
Rb) | CAGCACTTTCTAAGGCGACA | ||||
PGRMC1 | Fa) | AGGAGTGAGGTCGGAAAGGT | 165 | 59 | NM 001075133 |
Rb) | ATCAATGGCAAGGTGTTCG | ||||
PGRMC2 | Fa) | CCAGAGGACTGGCAACATTT | 167 | 56 | NM 001099060 |
Rb) | ACGGTTCTTCCCCTGGTTT | ||||
GAPDH | Fa) | CCACTTGATGTTGGCAGGAT | 66 | 59 | XM 001252511 |
Rb) | GAAGCTCGTCATCAATGGAAA |
a) Forward, b) Reverse.