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PI3K immunohistochemical staining (× 400). (A) Hemangiosarcoma (HSA) showing weak (1+) cytoplasmic and nuclear expression (arrowheads) in Case 1. (B) HSA showing strong (2+) cytoplasmic and nuclear expression (arrowheads) in Case 6. (C) A normal spleen showing no expression (control), which may reflect the presence of PI3K-expressing leukocytes (arrows), in Case 11. Normal endothelial cells show no expression. Bar, 60 µm.
