Table 1. Primary antibodies used in immunohistochemical analysis.
| Primary antibody | Host (clone) | Dilution | Company |
|---|---|---|---|
| c-kit | Rabbit | 1:50 | Santa Cruz |
| VEGFR-2 | Mouse | 1:250 | Santa Cruz |
| PDGFR-2 | Rabbit | 1:1,000 | Cell Signaling |
| PI3K | Rabbit | 1:500 | Upstate |
| phosphor-Akt | Rabbit (D9E) | 1:50 | Cell Signaling |
| phosphor-m-TOR | Rabbit (49F9) | 1:500 | Cell Signaling |
| MEK1 | Mouse (25/MEK1) | 1:1,000 | BD Transduction Laboratories |
| MEK2 | Mouse (96/MEK2) | 1:1,000 | BD Transduction Laboratories |
| phospho-p44/42 MAPK (ERK1/2) | Rabbit | 1:100 | Cell Signaling |