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. 2016 May 18;7:11716. doi: 10.1038/ncomms11716

Figure 6. Macrophage ABHD5 suppresses ROS-dependent C/EBPɛ expression.

Figure 6

(a) The ROS production in the PLKO or ABHD5-KD RAW cells treated with NAC (20 mM), GSH (10 mM) or vehicle control PBS for 24 h were measured. The data represent means±s.e.m., (n=5, **P<0.01, Student's t-test). (b) The ROS production in the pCDNA3.1 or pCDNA-ABHD5-transfected RAW cells. The data represent means±s.e.m., (n=5, **P<0.01, Student's t-test). (c) ABHD5, C/EBPɛ and SRM mRNA levels in the RAW cells as described in a. The data represent means±s.e.m., (n=4, **P<0.01, Student's t-test). (d) Immunoblotting assay of C/EBPɛ and SRM in the RAW cells described in a. (e) The ChIP assay was performed to determine the interaction between C/EBPɛ protein and SRM promoter in the RAW cells described in a. (f) Supernatant spermidine levels in the RAW cells described in a. The data represent means±s.e.m., (n=4, **P<0.01, Student's t-test). (g) Tumour volumes of the mice that were subcutaneously inoculated with CT-26 cells in the thighs and treated with the CM from the RAW cells described in a for 2 weeks. The data represent means±s.e.m., (n=6, **P<0.01, Student's t-test). NS, not significant.