Figure 6. P21^WAF1/CIP1 overexpression impairs the survival of KRAS-mutant cancer cells.

(a) T29Kt1 and T29 cells were transfected with p21 cDNA plasmid or pcDNA 3.1 empty vector. Cell viability was measured 48 h after transfection. Data represent the mean±s.d. from three independent experiments. The efficiency of p21 overexpression was examined by immunoblotting. (b) T29Kt1 and T29 cells were transfected with p21 cDNA plasmid or pcDNA 3.1 empty vector. The percentage of apoptotic cells (Annexin positive) was determined by Annexin-V and propidium iodide staining. (c) Cell viability was measured in various cancer cells after p21 cDNA transfection. The dots represent the relative cell viability. The bars indicate the means. Student's t-tests were performed between the MUT and WT groups; **P<0.01; ***P<0.001. The efficiency of p21 overexpression in representative cells were examined by immunoblotting. (d) T29Kt1, T29 cells and different cancer cell lines were treated with SAHA or vehicle control for 48 h. Cell viability was measured. The columns indicate the means, and the bars indicate the s.d. The dots represent relative cell viability. Student's t-tests were performed between the MUT and WT groups; **P<0.01; ***P<0.001. MUT, mutant; WT, wild type.