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. 2016 May 18;7:11635. doi: 10.1038/ncomms11635

Figure 3. The essential role of HIF-1α - PDK1 axis in hypoxia-induced glycolytic reprogramming.

Figure 3

(a,b) (i) The relative expression of Pdk1 or Pdk3 in RAW 264.7 cells with gene silencing by siRNA. (ii) OCR of RAW 264.7 cells treated with Pdk1 or Pdk3 siRNA of under mild hypoxia (8% O2, 4 h-6% O2, 4 h-4% O2, 4 h). (c,d) OCR of TEPMs treated with DCA (10 mM), a chemical inhibitor of PDK, under mild hypoxia (8% O2, 4 h-6% O2, 4 h-4% O2, 4 h). All graphs indicate average with s.d. All experiments using XF24 were repeated three times. Student's t-test was performed to calculate P value. *P<0.05 versus control (negative control); †P<0.05 versus control (untreated); NS, not significant; wt TEPM, Tie2-Cre−/− HIF-1αflox/flox mice; HIF-1α KO, Tie2-Cre+/− HIF-1αflox/flox mice; DCA ; 21, 8, 6 and 4% O2 concentration, respectively.