Fig. 5.

Production of B cell chemotactic factors following viral infection and cytokine stimulation of cultured primary murine microglial cells and astrocytes. Primary murine microglial cell (black bars) or astrocyte (gray bars) cultures in 48-well plates (1 × 10⁵ cells/well) were infected with MCMV (MOI = 5) or stimulated with the indicated pro-inflammatory cytokine (i.e., TNF-α and IL-6, 20 ng/ml; IL-1β and IFN-γ, 10 ng/ml; IFN-α and IFN-β, 200 U/ml) for 48 h. Production of select B cell-attracting chemokines was assessed using ELISA. Pooled data are presented as mean ± SEM from three to four independent experiments