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. 2016 May 20;14:12. doi: 10.1186/s12964-016-0135-0

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© Nunes de Miranda et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Lyn/Ca²⁺/calcineurin-dependent signaling controls Ag-induced transcription of various inflammatory genes. BMMCs were preloaded and starved overnight with 0.15 μg/ml IgE. a Wt, Lyn-/-, SHIP1-/-, and dko BMMCs were left untreated (con) or stimulated with 20 ng/ml of Ag for 90 min. The amounts of Tnfsf9 mRNA, Tnip3 mRNA, Il1a mRNA, Il1b mRNA, and Stx11 mRNA were measured by RT-qPCR. A comparison of analyses of different independent cell cultures is depicted in Additional file 8: Figure S8. b SHIP1-/- BMMCs, pretreated with vehicle or 100 nM CsA for 30 min, were left unstimulated (con) or stimulated with 200 ng/ml Ag for 90 min. The amounts of Tnfsf9 mRNA, Tnip3 mRNA, Il1a mRNA, Il1b mRNA, and Stx11 mRNA were measured by RT-qPCR. Comparable results were obtained with cells from different BMMC cultures (n = 3 (a); n > 3 (b)