Figure 2. Robo receptors are required to control PSC morphology during larval development and MZ lineage tracing.

(a–f) Antp (red) and GFP (green) label PSC cells in control (col>GFP) (a–c) and in col>robo KD LGs (d–f). (a,d) L1 larvae, (b,e) L2 larvae and (c,f) early L3 larvae. (g,j) Antp (red) and GFP (green) label PSC cells in Gal80ts context; control (col>) (g,i) and in col>robo KD LGs (h,j). The temperature shift (18–29 °C) was performed in L1 (g,h) or L2 (i,j), and LGs were analysed in L3. (k–n) Antp (red) labels PSC cells (k,m) and GFP (green) labels MZ lineage traced cells in control (dome>Flp; ubi>FRT>GFP) (k,l) and in robo2^Ex33/+ heterozygous mutant (dome>Flp; ubi>FRT>GFP; robo2^Ex33/+) (m,n). Nuclei are labelled with 4,6-diamidino-2-phenylindole (blue) (a–n). Scale bars, 10 μm.