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. Author manuscript; available in PMC: 2016 Jul 28.
Published in final edited form as: Biochemistry. 2015 Jul 15;54(29):4542–4554. doi: 10.1021/acs.biochem.5b00584

Figure 5.

Figure 5

Representative growth curves for wild-type (WT), ΔcstB, and plasmid-complemented ΔcstB allelic S. aureus Newman strains on HHWm media in the presence of 0 mM and 0.2 mM NaHS added at t=0 h. (A) WT (black square), ΔcstB (blue circle) and ΔcstB:pCstB (green diamond) strains in the presence of 0 mM NaHS; WT (red square), ΔcstB (magenta circle) and ΔcstB:pCstB (wine diamond) strains in the presence of 0.2 mM NaHS. Note that both the WT and ΔcstB:pCstB strains exhibit a growth lag (≈7 h) in the presence of 0.2 mM NaHS; ΔcstB exhibits a more significant growth lag (≈9 h) in the presence of 0.2 mM NaHS. Also note that all the strains grow normally in the presence of 0 mM NaHS. (B) ΔcstB:pCstBC20S (black square), ΔcstB:pCstBC201S (blue circle) and ΔcstB: pCstBC408S (green diamond) strains in the presence of 0 mM NaHS; ΔcstB:pCstBC20S (red square), ΔcstB:pCstBC201S (magenta circle) and ΔcstB: pCstBC408S (wine diamond) strains in the presence of 0.2 mM NaHS. Note that all the three strains exhibit a more significant growth lag (≈9 h) in the presence of 0.2 mM NaHS. Also note that all the strains grow normally in the presence of 0 mM NaHS.