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Comparison of the thiosulfate transferase (TST) activities of various CstBs.a
| Enzyme |
Km
(x10−3 M) |
Vmax
(μmol SCN min−1 mg−1) |
kcat (s−1) |
kcat/Km
(x10−3 M− ·s−1) |
|---|---|---|---|---|
| CstB | 1.7 ± 0.2 | 10.4 ± 0.6 | 8.5 ± 0.5 | 5.0 ± 0.7 |
| C201S CstB | 2.2 ± 0.4 | 11.2 ± 0.9 | 9.2 ± 0.7 | 4.2 ± 0.8 |
| C408S CstB | NAb | NA | NA | NA |
| CstBPDO-RHD | NA | NA | NA | NA |
| CstBRhod | NA | NA | NA | NA |
| CstBPDO-RHD + CstBRhod | NA | NA | NA | NA |
Condition: 0.4 μM CstB (protomer), 50 mM KCN, 100 mM MES, pH 6.0, 25 °C.
NA, no activity observed.
