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Depletion of clathrin heavy chain reduces basal VEGFR2 and mature VEGFR1 levels. (A) Endothelial cells were subjected to scrambled (Scr) or clathrin heavy chain (CHC17)-specific siRNA duplexes, prior to cell lysis and processing for immunoblot analysis. (B) Quantification of basal VEGFR2, VEGFR1, NRP1, transferrin receptor (TfR), TGN46 and CHC17 levels upon depletion of clathrin heavy chain (CHC17). Error bars indicate ±s.e.m. (n=3). **P<0.01, ***P<0.001, ****P<0.0001.
