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. 2016 Apr 25;5(5):620–630. doi: 10.1242/bio.018119

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Phagocytosis triggers CLIC1 translocation to BMDC phagosomal membrane. (A-F) Immunofluorescence confocal microscopic images of resting CLIC1^+/+ BMDCs (A-C) or BMDCs phagocytosing IgG-opsonised zymosan particles (D-F), stained with antibodies to RhoA (green) or CLIC1 (red). (G-L) Images of resting CLIC1^−/− BMDCs (G-I) or BMDCs phagocytosing IgG-opsonised zymosan particles (J-L), stained with antibodies to RhoA (green) and CLIC1 (red). Both CLIC1 and RhoA appear on the phagosomal membrane after 5 min of phagocytosis in CLIC1^+/+ BMDCs (F, arrows). Only RhoA can be identified on the phagosomal membrane of CLIC1^−/− BMDCs (L, arrows). Scale bar: 10 µm.