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. 2016 Apr 25;5(5):620–630. doi: 10.1242/bio.018119

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — CLIC1^−/− BMDCs display impaired phagosome acidification. Live BMDCs that had undergone synchronised phagocytosis of an IgG-opsonised zymosan particle covalently coupled with a pH-sensitive fluorescent probe, in the presence or absence of IAA94 (100 μM), were monitored continuously for 30 min using an inverted Zeiss Axiovert 200 M microscope. (A) The phagosomal pH of CLIC1^−/− BMDCs was higher than that of CLIC1^+/+ BMDCs over the 30 min time course. (B) IAA94 treatment had no effect on the steady state phagosomal pH of CLIC1^−/− BMDCs, but impaired the acidification of CLIC1^+/+ BMDC phagosomes to the same level as that of CLIC1^−/− BMDCs. Data represents mean±s.e.m. analysed using two-way repeated-measures ANOVA or paired t-test respectively.