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. 2016 Apr 25;5(5):620–630. doi: 10.1242/bio.018119

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — IAA94 reduces CD4⁺ T-cell activation by MOG_1-125-pulsed CLIC1^+/+ but not CLIC1^−/− BMDCs. The proportion of activated (intracellular IFNγ-containing) MOG-specific 2D2 CD4⁺ T-cells after 16 h coculture with IAA94 (100 μM) treated or untreated BMDCs pulsed with (A) 1.25 pmoles of the short MOG_35-55 peptide or with (B) equimolar full length MOG_1-125. Fold increase in activated T-cells was calculated relatively to the no antigen control. Data representing mean±s.e.m. were analysed using two-way repeated-measures ANOVA.