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. Author manuscript; available in PMC: 2016 May 20.
Published in final edited form as: Cold Spring Harb Protoc. 2011 Dec 1;2011(12):1480–1482. doi: 10.1101/pdb.prot067009

Figure 1.

Figure 1

Hardware: (A) The stage, composed of x- and y-axis actuators and linear translation stages; (B) the camera; (C) the sample, a worm on an agar Petri dish; (D) a diffuser above a collimating Fresnel lens, to provide uniform illumination; (E) a high-intensity red light-emitting diode (LED) for illumination; (F) course and fine focusing knobs to focus the sample in view of the camera; (G) a rigid cage to center the illumination above the camera and eliminate vibrations caused by moving the stage.