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Experimental protocol for PC μCT. The inflation chamber (shown in Fig. 2) was immersed in a PBS bath, and the entire apparatus was placed on a rotating stage. An x-ray beam, produced by synchrotron radiation, was directed toward the ONH region. The x-ray detector was placed 2 meters behind the specimen. For PC μCT, a few millimeters of optic nerve were left on the eye.
