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. 2016 May 15;143(10):1688–1696. doi: 10.1242/dev.136606

Fig. 4.

Fig. 4.

Transiently amhc+ cardiomyocytes populate the ventricular apex. (A) Surface myocardium of a 7 dpf heart from an amhc:CreER; priZm animal treated with 4-HT at 3 dpf, displaying a non-red cardiomyocyte (white arrow) on the ventricular side of the AV junction (dashed line). (B) Surface myocardium of a 3 dpf heart from a vmhc:mCherry-NTR and amhc:EGFP animal. (C) Surface myocardium of a 2 dpf heart expressing vmhc:mCherry-NTR and immunostained for Amhc. Magnified region shows area of EGFP expression within the ventricle (V) near the AV junction (dashed line). Arrow indicates an Amhc+ cardiomyocyte in the ventricle. (D,E) Surface myocardium of 7 dpf amhc:CreER; priZm hearts from animals treated with 4-HT at 1 dpf or 5 dpf, respectively. (F) Percentage of ventricular surface area labeled in 7 dpf amhc:CreER; priZm hearts treated at 9 hpf (n=7), 18 hpf (n=8), 1 dpf (n=7), 2 dpf (n=7), 3 dpf (n=8) or 5 dpf (n=7) (means±s.e.m.). Asterisk indicates that the means are significantly heterogeneous (one-way ANOVA, P<0.05) and a significant difference exists between the indicated groupings (Tukey–Kramer test, P<0.05). (G,H) Surface and deep myocardium, respectively, of a 14 dpf amhc:CreER; priZm heart treated with 4-HT at 1 dpf. Cardiomyocytes of the primordial ventricular wall (prim) and trabecular muscle (trab) are indicated with an arrow. (I) Surface myocardium of a 42 dpf amhc:CreER; priZm heart treated with 4-HT at 1 dpf. A labeled region of the ventricular cortical myocardium (cort) is indicated with an arrow. Scale bars: 100 µm in I; 50 µm in A,B,D,E,G,H; 25 µm in C-C′.