Table 3. Indentified Botrytis cinerea proteins.
| Sequence Id | Putative Function | Singal P | MG | RR | rin | Predicted CAZy Class | ||||
|---|---|---|---|---|---|---|---|---|---|---|
|
| ||||||||||
| Pep | Spec | Pep | Spec | Pep | Spec | |||||
|
| ||||||||||
| BC1G_07319.1 | 1,3-beta glucanaseCWDE | 0.999 | Y | - | - | 3 | 3 | - | - | - |
| BC1G_10455.1 | 1,3-beta-glucanosyltransferase Gel1CWDE | 0.998 | Y | - | - | 4 | 19 | 3 | 4 | GH72 |
| BC1G_02623.1 | alpha-amylase | 0.994 | Y | - | - | 2 | 2 | 4 | 5 | GH13 |
| BC1G_08975.1 | alpha-fucosidase | 0.962 | Y | 3 | 3 | 2 | 2 | 4 | 4 | - |
| BC1G_12859.1 | alpha-glucosidase precursorCWDE | 1 | Y | - | - | 2 | 2 | 2 | 2 | - |
| BC1G_08372.1 | alpha-L-arabinofuranosidase ACWDE | 0.995 | Y | 3 | 3 | - | - | - | - | - |
| BC1G_04994.1 | alpha-L-arabinofuranosidaseCWDE | 0.999 | Y | - | - | - | - | 2 | 2 | GH54|CBM42 |
| BofuT4_P078550.1 | alpha-L-rhamnosidase BCWDE | 0.072 | N | - | - | - | - | 1 | 1 | GH78 |
| BC1G_03983.1 | beta-1,3 exoglucanaseCWDE | 0.27 | N | - | - | 2 | 2 | - | - | - |
| BC1G_14030.1 | beta-1-3-glucanosyltransferase | 1 | Y | 3 | 4 | - | - | 3 | 4 | GH72|CBM43 |
| BC1G_03567.1 | beta-galactosidaseCWDE | 0.998 | Y | 9 | 17 | 15 | 20 | 22 | 33 | GH35 |
| BC1G_10221.1 | beta-glucosidase 1 precursorCWDE | 0.816 | Y | 2 | 2 | - | - | - | - | GH3 |
| BC1G_02364.1 | beta-glucosidase, putativeCWDE | 0.991 | Y | 5 | 7 | - | - | - | - | GH3 |
| BC1G_05538.1 | beta-xylosidaseCWDE | 0.978 | Y | 1 | 1 | - | - | - | - | GH3 |
| BC1G_09079.1 | cell wall beta-1,3-endoglucanaseCWDE | 0.999 | Y | 3 | 3 | 4 | 6 | 8 | 11 | - |
| BC1G_14702.1 | cellobiohydrolase 1 catalytic domainCWDE | 1 | Y | 3 | 4 | - | - | 1 | 1 | - |
| BC1G_06035.1 | cellobiohydrolaseCWDE | 0.999 | Y | 12 | 21 | - | - | - | - | GH7 |
| BC1G_03188.1 | cellobiose dehydrogenaseCWDE | 0 | N | - | - | - | - | 7 | 8 | CBM1 |
| BC1G_10880.1 | cellobiose dehydrogenaseCWDE | 0.992 | Y | 17 | 57 | - | - | 12 | 19 | GH7 |
| BC1G_14012.1 | choline dehydrogenase, putative | 0.998 | Y | - | - | 2 | 2 | - | - | CBM1 |
| BC1G_08529.1 | cytochrome c | 0 | N | - | - | - | - | 2 | 2 | - |
| BC1G_00576.1 | endo-1,4 beta-D-xylanaseCWDE | 1 | Y | 2 | 2 | 2 | 3 | - | - | GH10 |
| BofuT4_P061530.1 | endo-1,4-beta-mannosidaseCWDE | 0.998 | Y | 1 | 1 | - | - | - | - | - |
| BofuT4_P124780.1 | endo-glucanase, putativeCWDE | 0.999 | Y | 1 | 3 | - | - | - | - | GH12 |
| BC1G_11143.1 | endo-polygalacturonase precursorCWDE | 1 | Y | 15 | 57 | 13 | 87 | 25 | 161 | GH28 |
| BofuT4_P024480.1 | esterase | 0.025 | N | - | - | 2 | 2 | 1 | 1 | - |
| BC1G_07822.1 | extracellular endo-glucanaseCWDE | 0.999 | Y | 3 | 3 | - | - | - | - | GH5 |
| BC1G_07496.1 | extracellular exo-polygalacturonase, putativeCWDE | 0 | N | 2 | 3 | - | - | - | - | GH28 |
| BC1G_00448.1 | extracellular serine-rich protein | 0.983 | Y | 3 | 3 | 2 | 2 | - | - | - |
| BofuT4_P151530.1 | formate dehydrogenase | 0.659 | Y | - | - | 1 | 1 | 3 | 4 | - |
| BC1G_04836.1 | fructose-bisphosphate aldolase | 0 | N | 4 | 4 | - | - | - | - | - |
| BofuT4_P119480.1 | GDSL lipase/acylhydrolase family protein | 0.988 | Y | 2 | 4 | - | - | - | - | - |
| BC1G_04151.1 | glucan 1,4-alpha-glucosidase | 0.912 | Y | 5 | 7 | 11 | 15 | 20 | 84 | CBM20|GH15 |
| BC1G_10788.1 | glucose oxidase | 0.997 | Y | 1 | 1 | - | - | - | - | - |
| BC1G_11898.1 | glucosidaseCWDE | 0.999 | Y | 3 | 5 | 5 | 11 | 4 | 5 | GH17 |
| BofuT4_P033100.1 | glutaminase GtaA | 0.992 | Y | - | - | 3 | 3 | - | - | - |
| BC1G_01204.1 | glyoxal oxidaseCWDE | 0.983 | Y | - | - | - | - | 4 | 4 | CBM18 |
| BC1G_01393.1 | hypothetical protein | 1 | Y | - | - | 2 | 2 | - | - | - |
| BC1G_02060.1 | hypothetical protein | 0.999 | Y | 1 | 2 | 2 | 3 | - | - | - |
| BC1G_02930.1 | hypothetical protein | 0 | N | - | - | 7 | 11 | - | - | - |
| BC1G_03976.1 | hypothetical protein | 0.959 | Y | - | - | - | - | 7 | 7 | - |
| BC1G_05298.1 | hypothetical protein | 0 | N | - | - | 2 | 2 | - | - | - |
| BC1G_05503.1 | hypothetical protein | 0 | N | 2 | 6 | - | - | 2 | 2 | - |
| BC1G_05523.1 | hypothetical protein | 0 | N | - | - | - | - | 5 | 5 | - |
| BC1G_05980.1 | hypothetical protein | 0.004 | N | - | - | 2 | 4 | - | - | - |
| BC1G_07073.1 | hypothetical protein | 1 | Y | - | - | 2 | 2 | - | - | - |
| BC1G_07275.1 | hypothetical protein | 1 | Y | - | - | - | - | 2 | 2 | - |
| BC1G_07903.1 | hypothetical protein | 0.001 | N | - | - | 3 | 3 | 2 | 2 | - |
| BC1G_08393.1 | hypothetical protein | 0.999 | Y | 3 | 5 | - | - | 2 | 4 | - |
| BC1G_08615.1 | hypothetical protein | 0.962 | Y | - | - | - | - | 2 | 2 | - |
| BC1G_08719.1 | hypothetical protein | 0.019 | N | 1 | 1 | - | - | 1 | 2 | - |
| BC1G_10630.1 | hypothetical protein | 1 | Y | 3 | 4 | - | - | - | - | - |
| BC1G_12374.1 | hypothetical protein | 0.999 | Y | 5 | 40 | 9 | 287 | 6 | 42 | - |
| BC1G_15041.1 | hypothetical protein | 0.988 | Y | 4 | 5 | - | - | 4 | 5 | - |
| BC1G_16040.1 | hypothetical protein | 0 | N | - | - | - | - | 1 | 1 | - |
| BofuT4_P129550.1 | hypothetical protein | 0.998 | Y | - | - | 2 | 2 | - | - | - |
| BC1G_12487.1 | hypothetical protein | 0 | N | - | - | 3 | 5 | 3 | 3 | - |
| BC1G_14129.1 | hypothetical protein | 0.997 | Y | 2 | 4 | 2 | 2 | 2 | 4 | - |
| BC1G_08553.1 | laccase 2CWDE | 0.999 | Y | 4 | 5 | 2 | 2 | 9 | 16 | - |
| BC1G_10329.1 | laccaseCWDE | 0.989 | Y | - | - | 4 | 6 | - | - | - |
| BofuT4_P040250.1 | L-PSP endoribonuclease family protein, putative | 0 | N | - | - | 2 | 2 | - | - | - |
| BC1G_00455.1 | mannosyl-oligosaccharide alpha-1,2-mannosidase precursorCWDE | 0.998 | Y | 4 | 5 | - | - | 2 | 2 | GH47 |
| BofuT4_P108020.1 | nucleoside diphosphate kinase | 0 | N | 1 | 6 | 1 | 3 | 2 | 3 | - |
| BC1G_12017.1 | pectin lyase A precursorCWDE | 0.998 | Y | - | - | 3 | 8 | 3 | 3 | PL1 |
| BofuT4_P131540.1 | pectin methyl esterase 1CWDE | 1 | Y | 5 | 9 | 4 | 6 | 6 | 12 | CE8 |
| BC1G_00617.1 | pectin methyl esteraseCWDE | 1 | Y | 2 | 4 | - | - | 2 | 5 | CE8 |
| BC1G_11144.1 | pectin methylesterase, putativeCWDE | 1 | Y | 2 | 4 | - | - | - | - | CE8 |
| BC1G_13367.1 | polygalacturonase 2 CWDE | 0.999 | Y | - | - | - | - | 2 | 3 | GH28 |
| BC1G_00896.1 | predicted protein | 1 | Y | 5 | 39 | 5 | 44 | 6 | 87 | - |
| BC1G_09084.1 | predicted protein | 1 | Y | - | - | 1 | 1 | - | - | - |
| BC1G_14009.1 | rhamnogalacturonan acetylesteraseCWDE | 0.983 | Y | - | - | 3 | 10 | - | - | - |
| BofuT4_P124300.1 | rhamnogalacturonan hydrolase CWDE | 1 | Y | 1 | 1 | - | - | 1 | 1 | GH28 |
| BC1G_06836.1 | serine protease | 0.999 | Y | - | - | 3 | 4 | - | - | - |
| BC1G_02163.1 | SnodProt1CWDE | 0.996 | Y | 13 | 65 | 27 | 253 | 19 | 154 | - |
| BC1G_14403.1 | thioredoxin | 0 | N | 2 | 2 | 3 | 3 | 2 | 3 | - |
| BofuT4_P021640.1 | transaldolase 1 | 0 | N | 2 | 2 | 2 | 2 | 1 | 5 | - |
| BC1G_00979.1 | tripeptidyl-peptidase 1 precursor | 0.015 | N | 1 | 1 | - | - | 1 | 2 | - |
| BC1G_02944.1 | tripeptidyl-peptidase 1 precursor | 0.999 | Y | - | - | - | - | 2 | 2 | - |
| BC1G_10797.1 | xylosidase : arabinofuranosidaseCWDE | 0.997 | Y | 2 | 2 | - | - | 2 | 2 | - |
Sequence id: Identification number provided by the Broad Institute BO5.10 database
Putative function: was assigned based on sequence similarity whne blasted using NCBI non redundant database
Signal P: prediction value using algoritm SignalP3.0 server (http://www.cbs.dtu.dk/services/SignalP/); Y, signal peptide predicted. N,no signal peptide predicted.
Peptides: Total number of non-redundant peptides above a threshold score of 54 matched to the fungal protein in that infected tomato fruit
Spectra: Total number of spectra matched to proteins in all replicates in that infected tomato fruit
CAZy families: Predicted with CAZYmes Analysis Toolkit (CAT) from the BESC KnowledgeBase website (http://bobcat.ornl.gov/besc/index.jsp)