Table 1.
Primers, amplicon size and amplification conditions for the PCR assays.
| Gene target | Sequence (5′–3′) | Determinant | Amplicon | Amplification conditions | Reference |
|---|---|---|---|---|---|
| 23S rRNA | F = GGGGAACCCACTATCTTTAGTC R = GGGCCTTTCCAGACCGCTTCA |
Genus Listeria | 239 bp | 95 °C (1 min), 62 °C (1 min), 72 °C (1 min) | Hudson et al.26 |
| D1 | F = CGATATTTTATCTACTTTGTCA R = TTGCTCCAAAGCAGGGCAT |
Division I or III | 214 bp | 95 °C (30 s), 56 °C (30 s), 72 °C (1 min) | Borucki and Call27 |
| D2 | F = GCGGAGAAAGCTATCGCA R = TTGTTCAAACATAGGGCTA |
Division II | 140 bp | 95 °C (30 s), 56 °C (30 s), 72 °C (1 min) | Borucki and Call27 |
| ORF2110 | F = AGTGGACAATTGATTGGTGAA R = CATCCATCCCTTACTTTGGAC |
Serotype 4b | 597 bp | 95 °C (1 min), 60 °C (1 min), 72 °C (1 min) | Doumith et al.28 |
| lmo0737 | F = AGGGCTTCAAGGACTTACCC R = ACGATTTCTGCTTGCCATTC |
L. monocytogenes serovars 1/2a, 1/2c, 3a and 3c | 691 bp | 95 °C (1 min), 60 °C (1 min), 72 °C(1 min) | Doumith et al.28 |
| lmo1118 | F = AGGGGTCTTAAATCCTGGAA R = CGGCTTGTTCGGCATACTTA |
L. monocytogenes serovars 1/2c and 3c | 906 bp | 95 °C (1 min), 60 °C (1 min), 72 °C(1 min) | Doumith et al.28 |
| ORF2819 | F = AGCAAAATGCCAAAACTCGT R = CATCACTAAAGCCTCCCATTG |
L. monocytogenes serovars 1/2b, 3b, 4b, 4d, and 4e | 471 bp | 95 °C (1 min), 60 °C (1 min), 72 °C(1 min) | Doumith et al.28 |
| Hly | F = GCCTGCAAGTCCTAAGACGCCAATC R = CTTGCAACTGCTCTTTAGTAACAGC |
Listeriolysin O | 706 bp | 95 °C(1 min), 62 °C (1 min), 72 °C(1 min) | Hudson et al.26 |