Fig. 2.

Colony PCR to detect the presence of pHF5Ca integrated into the chromosomal DNA of Xac. Subsequent to electrotransformation of Xac with the expression vector pHF5Ca, six kan^R mutants were selected and subjected to colony PCR using the primers PBS1479F/R specific for the amplification of the tap1479 (approximately 640 bp). The PCR amplicons were visualized after migration through a 0.7% agarose gel. Lane: 1, WT Xac (negative control); 2–7, the six selected mutants; 8, PCR using pHF5Ca as the DNA template (positive control); and 9, DNA molecular weight marker (1 kb DNA ladder, NEB).