Fig. 3.

The integration of the TAP expression system into the chromosome of Xac does not alter its pathogenicity. Two independently selected mutants of Xac amy::pHF5Ca (1 and 2) were inoculated into leaves of sweet orange Bahia alongside the wild type strain (Xac wt) and a sample of NYG-medium (negative control). A map of the inoculation is shown on the right-hand side of the figure. Pictures were taken immediately after inoculation (A–C) and also 7 days post-inoculation (D–F). The test was conducted in triplicate (A–C).