Figure 5. A synergistic response to the combination of venetoclax and dexamethasone in plasma cells from myeloma patients.

Two million total cells from buffy coats were re-suspended in medium for culture, while the remaining cells were subjected to CD138+ cell isolation. Cells were treated with the indicated concentrations of venetoclax either alone or in combination with 0.5 μM dexamethasone for 24 h. Apoptosis was determined by staining with antibodies against CD38, CD45, and Annexin-V-FITC. Lysates were obtained from isolated CD138+ cells and protein subjected to co-immunoprecipitation and Western blot analysis as described in Fig. 1.