Table 1.

Characteristics of glycolytic enzymes of Diplonema papillatum. n.d., not detected.

	PTS
enzyme	D. papillatum	T. cruzi	monophylya	activityb
1. HK	PTS2	PTS2	yes (0.85)	0.92c
1. GLK	PTS1	PTS1	yes (0.96)
2. PGI	PTS1	PTS1	yes (0.93)	39.1
3. PFK	PTS1/n.d.	PTS1	no (0.99)	n.d.
3. FBPase (gluconeogenic)	PTS1	PTS1	no (1.00)	4.7
4. FBPA	PTS2	PTS2	yes (1.00)d	14.2
5. TIM	PTS1	n.d.	unresolved	123.2
6. GAPDH	n.d.	PTS1/n.d.	no (0.90)	48.5
7. PGK	PTS1	PTS1/n.d.	yes (0.81)	103.7
8. PGAM	n.d.	n.d.	no (1.00)	2100
9. ENO	n.d.	n.d.	yes (0.99)	3.7
10. PK	n.d.	n.d.	yes (0.98)	15.1

^aMonophyly of D. papillatum and kinetoplastids with posterior probability in parenthesis based on the relevant protein phylogeny (figure 1; electronic supplementary material, figures).

^bnmol min⁻¹ mg⁻¹ protein of the D. papillatum cell extract (see also electronic supplementary material).

^cThe activities of HK (high-affinity type) and GLK (low-affinity type) are indistinguishable.

^dRef [12].