Figure 9. Partitioning of transport proteins into dense FG nup phase: summary of experimental results.
Experimentally, partitioning of the transport proteins (TP) complexes with various cargoes into the dense FG nup phase depends on the cargo size and the overall interaction strength of the complex with the FG nups. Both Importin- (vertebrate homologue of Kap-) and NTF2 penetrate the dense phase, but the Importin- with either medium (IBB-GFP) or large (IBB-MBP-GFP) cargo does not. However, the very large complex of four Importin- complexed with four ZsGreen proteins partitions into the dense phase. The results are for the dense phase of TtNup98 of Tetrahymena Thermophila recombinantly expressed in bacteria, adapted from Schmidt and Görlich (Schmidt and Görlich, 2015).