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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Am J Transplant. 2016 Mar 14;16(6):1917–1922. doi: 10.1111/ajt.13695

Figure 2. Direct and indirect xenoresponses of baboon PBMCs.

Figure 2

Peripheral blood mononuclear cells (PBMCs) from naïve (Panel A) and transplanted (Panel B) baboons were isolated and cultured in vitro with intact xenogeneic pig irradiated PBMCs (direct xenoresponse, grey bars) or sonicates from pig PBMCs (indirect xenoresponse, black bars). Baboon PBMCs cultured with medium only were used as controls (white bars). The frequencies of PBMCs producing type 1 cytokines (γIFN- and IL-2) or type 2 cytokines (IL-4 and IL-5) were determined by ELISPOT. The results are expressed as numbers of cytokine-producing cells per million PBMCs. The results are representative of three baboons tested individually.