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. Author manuscript; available in PMC: 2017 May 19.
Published in final edited form as: Cell. 2016 Apr 28;165(5):1092–1105. doi: 10.1016/j.cell.2016.04.009

Figure 1. Fibroblasts Induce Cancer Platinum Resistance.

Figure 1

(A–D) Effect of fibroblasts on cisplatin resistance to OC8 (A and B) or A2780 (C and D) in vivo. Tumor cells or tumor plus fibroblasts were inoculated subcutaneously into mice and treated with or without cisplatin every 3 days for three cycles. Tumor volume was monitored (A and C) (mean ± SEM; n = 5). Apoptosis was determined by TUNEL staining. Representative images and TUNEL-positive cells (percentage of control) are shown (B and D). Scale bar, 40 μm. Mean ± SD; n = 10 random fields from three sections. *p < 0.05.

(E) Cisplatin-induced apoptosis on GFP+ tumor cells in the mixed co-culture of A2780-GFP and fibroblasts. Representative FACS data are shown (left). Mean ± SD, n = 3. *p < 0.05.

(F–H) Cisplatin-induced apoptosis in A2780 (F), OC8 (G), or NIH: OVCAR3 (H) co-cultured with fibroblasts from different ovarian cancer patients in the Transwell. Mean ± SD; n = 3, *p < 0.05.

(I and J) Cisplatin-induced apoptosis on A2780 (I) or OC8 (J) cultured in fibroblast medium. Mean ± SD; n = 3, *p < 0.05.

See also Figure S1 and Table S2.