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. Author manuscript; available in PMC: 2017 May 19.
Published in final edited form as: Cell. 2016 May 5;165(5):1171–1181. doi: 10.1016/j.cell.2016.04.018

Figure 4. A cold sensitive allele of the NME1 P3 domain confers cold-sensitive telomere shortening.

Figure 4

Telomere length analyses in strains harboring the indicated TLC1 alleles. Lanes 1–4: TLC1; lanes 5–8: tlc1Δ; lane 9–12: tlc1-ΔL; lanes 13–16: tlc1-ΔL; lanes 17–20: tlc1-P3NME1; lanes 21–24: tlc1-P3nme1-11. Schematics of stem IVc structures and color coding as in Figure 3 (see also Figure S4A). +: Strain carried a wt TLC1 gene on a URA3 plasmid. Black triangles: growth of cells after loss of the wt TLC1 gene at 30°C for 30 and 110 generations. Lanes with the blue star: strains were grown at 18°C for 110 generations. The red/blue bar at bottom indicates the temperatures the respective strain was grown at.