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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Hepatology. 2016 Jan 21;63(6):1783–1795. doi: 10.1002/hep.28380

Figure 5. Potent anti-HCV suppression by RA and its enhancement of ISG expression.

Figure 5

A. Huh7 HCV-SGR cells were treated with ATRA(0.1μM) or IFNβ(10IU/ml) for 72 hours. Extracts were subjected to IB analysis of the indicated proteins. B. RT-qPCR analysis of RNA extracted from PHH pretreated with ATRA(0.1μM) or vehicle for 16 hours followed by IFNβ(100IU/ml) for 8 hours. C and E. RT-PCR for the ISGs expression analysis. Huh7 cells stably expressing ADH1B were transfected with HCV-PAMP-RNA(1μg/5×105cells) for indicated hours(C) or treated with IFNβ(100IU/ml) for 8 hours(E). *p<0.01. D and F. IB analysis of the ISGs expression. Huh7 cells stably expressing ADH1B were transfected with HCV PAMP RNA(0, 2, 3, 4 μg/5×105cells) for 24 hours(D) or treated with IFNβ(0, 1, 10, 100IU/ml)(F).