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. Author manuscript; available in PMC: 2017 May 20.
Published in final edited form as: ACS Synth Biol. 2016 Mar 1;5(5):395–404. doi: 10.1021/acssynbio.5b00266

Figure 1.

Figure 1

Panel of core promoters enables a wide range of mammalian gene expression in the uninduced state. (a) Schematic of expression cassette for core promoter evaluation. “PolyA” indicates the bovine growth hormone (BGH) polyadenylation signal. (b–e) HEK 293T cells were transiently transfected with plasmids encoding various core promoters driving the expression of (b) Gluc or (c–e) sfGFP. Each plasmid also encodes for dsRed-Express expressed from a constitutive CMV promoter. (b) Gluc enzymatic activity in culture supernatant reacted with coelenterazine substrate. (c) sfGFP median fluorescence intensity (MFI) among transfected (dsRed+ gated) cells. (d) Percentage of transfected cells that are sfGFP+. (e) sfGFP MFI among dsRed+ and sfGFP+ gated cells. Values shown are the means of triplicates with error bars indicating ± 1 standard deviation (s.d.).