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. Author manuscript; available in PMC: 2017 May 20.
Published in final edited form as: ACS Chem Biol. 2016 Mar 4;11(5):1362–1371. doi: 10.1021/acschembio.5b00724

Figure 5. SEC-MALS analysis of full-length and N-terminal truncated TcVSP1s.

Figure 5

(A) The top panel shows the truncation positions. (B) SEC-MALS results obtained in the absence and presence of 0.1 mM CaCl2. The horizontal thin line segments represent the calculated molar masses and their corresponding molecular weights (left ordinate axis). The dashed grey line and the solid black line correspond to the normalized UV absorbance at 280 nm (right ordinate axis) and the intensity of light scattering, respectively.