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. Author manuscript; available in PMC: 2017 May 19.
Published in final edited form as: Cell. 2016 May 5;165(5):1106–1119. doi: 10.1016/j.cell.2016.04.015

Figure 6. OMV Production is Essential for E. coli-induced Caspase-11 Activation.

Figure 6

(A) Cell death and secretion of IL-1β and IL-1α by unprimed BMDM stimulated with wild-type or indicated hypovesiculating strains of E. coli at an MOI of 25 for 16 h.

(B) Cell death and secretion of IL-1β by wild-type or caspase-11−/− BMDM transfected with lysates from wild-type or indicated hypovesiculating strains of E. coli or stimulated with E. coli or poly(dA:dT) for 16 h.

(C) LPS levels, as determined by LAL assay, in the cytosol and residual fractions of BMDM infected with wild-type or indicated hypovesiculating strains of E. coli for 4 h.

(D-E) Secretion of IL-1β and cell death in BMDM infected with wild-type or indicated hypovesiculating strains or their corresponding complements expressing the deleted gene on a plasmid (blue bars) at an MOI of 25 for 16 h.

(F) Cell death in HeLa cells stimulated with wild-type or indicated hypovesiculating strains of E. coli at an MOI of 300 for 16 h.

Data are presented as mean±SEM of one experiment representative of three experiments for AB and two experiments for C-F. See also Figure S5.