Figure 6. Swollen nuclei that contain cPla2 attract leukocytes to cell corpses.
(A) Left panel, scheme of experimental design. Amputated zebrafish larvae were immersed in isotonic bathing solution to inhibit endogenous, wound-induced inflammation. Amputation wounds were exposed to differently conditioned HeLa cell suspensions. Nuclear swelling was initiated by digitonin-permeabilization and blocked by PVP-supplementation as described. Right panel, average number of leukocytes recruited to the wound margin within 60 min after cell-exposure of amputated tail fins at indicated conditions (table). Note that only swollen (condition #5), but not nonswollen nuclei (condition #3 and #4) that contain cPla2-mK2 attract leukocytes to cell corpses at permissive [Ca2+]. Error bars, SEM. ***, t-test p<0.0005. n, number of animals.
(B) Transmitted light images of zebrafish larvae exposed to permeabilized cell corpses with non-swollen (top, condition #4) or swollen (bottom, condition #5) nuclei that contain cPla2-mK2. Representative leukocyte tracks are superimposed and color-coded. Please refer to Methods for details. Scale bars, 100 μm.