Skip to main content
. 2016 May 19;62(4):572–585. doi: 10.1016/j.molcel.2016.04.016

Figure 1.

Figure 1

Distal diUbLys48 ABP Labels SARS PLpro

(A) Ub-conjugate cleavage in TNF-α-treated HeLa cell lysates by SARS and MERS PLpro. Dotted lines added for clarity.

(B and C) Schematics of (B) Ub chain recognition by SARS PLpro and (C) recognition and cleavage of biotin-tagged triUbLys48.

(D) Cleavage of biotin-triUbLys48 by SARS PLpro. Cleavage intermediates detected by avidin-HRP reveal biotin on the diUb product.

(E) Qualitative labeling of SARS PLpro by Ub-ABPs (cartoons at bottom with red stars indicate warhead positions).

(F) Quantitative labeling of SARS PLpro by Ub-ABPs indicating percent of SARS PLpro labeled as derived from gels in Figure S2C. Error bars represent ±SEM.

See also Figures S1 and S2.