Table 1.
Summary of phenotypic conversion upon passage experiments
Inoculated host, year | # Trees (# Leaves)a | Inoculum; duration in host | Phenotypesf | Total | |
---|---|---|---|---|---|
wt | nwt | ||||
Petri plate control | n/a | Pr-710, Pr-745, Pr-1556, Pr-1557, ND886 (wt, foliar hosts)b ; n/a | 348 | 0 | 348 |
n/a | Pr-102 (nwt, oak)c ; n/a | 71 | 29 | 100 | |
Canyon live oak, 2010 | 8 | Pr-710 and Pr-745 (wt, bay); 20 weeks | 37 | 25 | 62 |
10 | Pr-710 and Pr-745 (wt, bay); 40 weeks | 23 | 35 | 58 | |
Shreve oak, 2010 | 2 | Pr-710 and Pr-745 (wt, bay); 20 weeks | 6 | 2 | 8 |
Canyon live oak, 2012 | 4 | Pr-1556 and Pr-1557 (wt, bay); 20 weeks | 27d | 0 | 27 |
California bay, 2012 | 1 (9) | Pr-745 (wt, bay); 20 weeks | 9 | 0 | 9 |
California bay, 2013 | 3 (27) | ND886 (wt, camellia); 4 to 25 weekse | 20 | 0 | 20 |
3 (27) | Pr-102 (nwt, oak); 4 to 25 weekse | 16 | 2 | 18 |
aThe total number of trees and the total number of leaves re-isolation were attempted. The pathogen was inoculated on oak stems or California bay leaves
bcolony morphology of five wt isolates grown on Petri plates was scored. 24, 24, 100, 100 and 100 replicates were made for Pr-710, Pr-745, Pr-1556, Pr-1557, and ND886, respectively
cPr-102 displaying wt colony morphology was used as inoculum. The 71 subcultures showing wt morphology were expected to display nwt colony morphology at a certain percentage upon subsequent subculturing
dMost of the tested wt isolates displayed nwt after passage through race tubes
ere-isolation was made 4, 12 and 25 weeks post-inoculation
fCriteria for scoring of wt/nwt are described in Evaluation of colony morphology in Methods