Table 3.
Primers of qRT-PCR assay used for eight RNA-seq libraries in this study
| Gene_id | Amplicon size (bp)a | Forward primer (5′- > 3′) | Reverse primer (5′- > 3′) |
|---|---|---|---|
| HVU014442.1 | 142 | ATTTCTTCGACTGGGGCCTG | ATGACCTTGCCGTCGATCTC |
| HVU014441.1 | 190 | CAGGAGCCTGAGTAGATGCG | AATGAGAGGCCGACCACAAG |
| HVU029679.1 | 194 | AAGAAACTGATCCGAGGCGG | ATTCGTCCGGCCCGTATTTT |
| HVU021835.1 | 180 | GCGGCTATATCCCACCTTCC | AAGAGCTGAGGTGAAGCGAC |
| HVU015180.1 | 208 | TGGAGAAACGGATCGAAGCC | TGCAGCCCAGCTAGAAAAGG |
| HVU021802.1 | 206 | CCTTCACCTCCAGGAACGTC | CACTCCGATTCCACTTGGCT |
| HVU025938.1 | 180 | TGTCACCGCTGAACCAATCA | TTCACTGGGATTCACGGACG |
| HVU026759.1 | 86 | GAAGTCGCGCGAATCTGTTC | GAGCAGAGCAGCCAGATCAA |
| HVU015112.1 | 215 | AAGATCCCAACAAGGCGAGG | CCTGGCTTGCTCCTCTTTGA |
| HVU001215.1 | 229 | AAGCATGCCGTCTTACACCA | GGCCATCGGAGAGTGCATTA |
| HVU012352.1 | 163 | TGGCGTGTCAGCTGAGATTT | GGCCGTGAAGGACCAAAAAC |
| HVU033128.1 | 214 | CCATCAGACTGTGGCGTCTT | GAACCCTCTGCGCATAGACA |
| HVU023469.1 | 244 | AGCACTACTACGGCACCAAC | CTGAAACCGGCGTACTCCTT |
| HVU007307.1 | 216 | AGATGGGGTGCGAACTTGAG | CAGGGACAGGACCATCCAAC |
| HVU011039.1 | 115 | GGTGATCATGCGTGTGTTCG | CTTCTTGGCCGAGTCCTTGT |
| HVU033209.1 | 234 | AGCCCAAACCTACCAAGCTC | ATCATAGCGCAGGAGCCATC |
| HVU000918.1 | 87 | GTAGCTATCCACGGTCACGG | TGGAACTGTAAGCGTCCACC |
| HVU007765.1 | 208 | CGCATGCTGATGGAAAAGGG | ATTGCACCGCACTCAACAAC |
| HVU006724.1 | 135 | TTTGTGTGGGGAGGTCGATG | CATGCACTCTTCGGTGACCT |
| HVU016310.1 | 245 | TGAGGATGAAGCGAGTGCAG | TGGGGACTAGCACGCAAAAT |
aThe amplicon lengths for all the genes are within 70–250 bp range to satisfy the requirement for the primer pairs used for real time RT-PCR analysis using SYBR green according to manufacturer’s guide