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. 2016 May 21;18:111. doi: 10.1186/s13075-016-1018-x

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© Roggenbuck et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Reactivity of human monoclonal anti-beta2 glycoprotein I (aß2GPI) antibodies MBB2 (a) and HCAL (b) with phospholipids (PL) and PL-binding proteins by line immunoassay (LIA): MBB2 (0.1 mg/L) and HCAL (0.02 mg/L) were run in the LIA alone or together with serum, ß2GPI, blood donor serum, and bovine serum albumin (BSA). As the positive reaction control a mixture of human IgG and IgM was immobilized. a 1, 0.1 mg/L aß2GPI MBB2; 2, 0.1 mg/L aß2GPI MBB2 + 10 mg/L ß2GPI; 3, 0.1 mg/L aß2GPI MBB2 + 10 mg/L BSA; 4, 0.1 mg/L aß2GPI MBB2 + 30 μL serum (1/33); and 5, 30 μL serum (1/33). b 1, 0.02 mg/L aß2GPI HCAL; 2, 0.02 mg/L aß2GPI HCAL + 10 mg/L ß2GPI; 3, 0.02 mg/L aß2GPI HCAL + 10 mg/L BSA; 4, 0.02 mg/L aß2GPI HCAL + 30 μL serum (1/33); and 5, 30 μL serum (1/33)