Table 2.
Bacterial strains and plasmids and their relevant characteristics
| Strains & Plasmids | Relevant characteristicsa | Source or reference |
|---|---|---|
| Escherichia coli strain | ||
| DH5α | F- 80dlacZ, ΔM15, Δ(lacZYA-argF)U169, endA1, recA1, hsdR17(rK–mK+), deoR, thi-1, supE44, gyrA96, relA1 λ- | Invitrogen, CA, USA |
| Yeast strain | ||
| Saccharomyces cerevisiae AH109 | MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2 : : GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2, URA3 : : MEL1UAS-MEL1TATA-lacZ | [61] |
| Erwinia amylovora strains | ||
| Ea1189 | Wild type | [62] |
| Ea1189∆hrcU | hrcU deletion mutant, CmR | This study |
| Ea1189∆hrpP | hrpP deletion mutant, CmR | This study |
| Plasmids | ||
| pBBR1-MCS3 | TcR, broad host-range cloning vector | [54] |
| pGADT7 | LEU2, AmpR, Y2H activation vector | Clontech, CA, USA |
| pGBKT7 | TRP1, KmR, Y2H bait vector | Clontech, CA, USA |
| pLRT201 | AmpR, pMJH20 expressing DspE(1-737)-CyaA | [38] |
| pMJH20 | AmpR, pWSK29 containing codons 2 to 406 of CyaA | [63] |
| pRRM1 | TcR, pBBR1-MCS3 containing hrcU | This study |
| pRRM2 | TcR, pBBR1-MCS3 containing hrcU N266A | This study |
| pRRM3 | AmpR, pGADT7 containing hrpP | This study |
| pRRM4 | AmpR, pGADT7 containing hrpJ | This study |
| pRRM5 | KmR, pGBKT7 containing hrcU | This study |
| pRRM6 | KmR, pGBKT7 containing hrcU N266A | This study |
| pRRM7 | KmR, pGBKT7 containing hrcU 209-360 | This study |
| pRRM8 | KmR, pGBKT7 containing hrcU 209-360, N266A | This study |
| pRRM9 | TcR, pAlter-Ex1 containing hrcU | This study |
| pRRM10 | AmpR, pAlter-Ex1 containing hrcU N266A | This study |
| pAlter-Ex1 | TcR, mutagenesis vector | Promega, WI, USA |
| pKD3 | AmpR, CmR mutagenesis cassette template | [59] |
| pKD46 | AmpR, expresses λ red recombinase | [59] |
aCmR, TcR, AmpR, KmR indicates resistance to chloramphenicol, oxytetracycline, ampicillin and kanamycin