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. 2016 May 20;17:381. doi: 10.1186/s12864-016-2731-1

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© Shu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The effects of Aβ(1–42) peptide on 5hmC level in vitro. a-b Dot-blot assay shows Aβ treatment significantly decreased total 5hmC levels in a dose-dependent manner in HEK293ft cells. Aβ at 1-μM concentration is the most effective at decreasing 5hmC levels (*p < 0.05;** p < 0.01, unpaired t-test). c-f The cultured aNSCs are positive for neural stem cell markers Nestin (d) and SOX2 (e). g The representative images of dot-blot assay of Aβ treatment on 5hmC level in aNSCs. h The quantification result indicates Aβ at 1-μM concentration significantly decreases 5hmC level in aNSCs (*p < 0.05;** p < 0.01, unpaired t-test). i-j Dot-blot assay indicates that the global level of 5hmC in cortex is significantly higher than in HEK293ft cells and aNSCs (*p < 0.05;** p < 0.01, unpaired t-test)