Table 3.
Results of real-time PCR (DHI, DH2 and DH3) for MAP detection
| Sample | Primer set | ||
|---|---|---|---|
| DH1F-DH1Ra (IS900-69 bp) | DH2F-DH2Ra (IS900-65 bp) | DH3F-DH3Ra (F57 gene-80 bp) | |
| A | + + +b | + + + | + + − |
| B1 | + + + | + + + | + − − |
| B3 | + + + | + + + | + − − |
| C | + + + | + + + | - - - |
| D | + + − | + + + | - - - |
| E | + + + | + + + | + + − |
| F | + + + | + + + | - - - |
Tests performed in three independent PCR reactions on DNA extracted from seven samples (A, B1, B3, C, D, E, F) obtained by joining the corresponding five sampling units
aPrimers described by Herthnek and Bölske (2006)
bPCR positivity (+) was confirmed by the sequencing of at least one PCR product per reaction replicates. Percent of similarity with reference MAP sequences in NCBI Genebank was at least 98 %