Fig. 4.

Analysis of oxidized products generated from cellulose by action of PsLPMOs. a HPAEC chromatograms of the oligosaccharides products released by action of PsLPMOA. b HPAEC chromatograms of the oligosaccharides products released by action of PsLPMOB. The peak annotations are based on comparison with oligosaccharide standards oxidized at the C1 position (DP2ox-DP5ox). Peaks eluting at 27, 37, and 41 min are annotated with dotted lines. Controls ascorbate (gray line), PsLPMO with PASC for 24 h (green line) or 72 h (purple line). Reaction samples PASC (0.1 %) with 4.4 µM PsLPMOs in the presence of 1 mM ascorbate, at 50 °C for 24 h (blue line) or 72 h (red line), and same reaction followed by the incubation with NcCDHIIA at 50 °C for 16 h (black line)