Fig. 1.

MSC clinical expansion. From ±50 mL of initial fresh BM, MNC cells were isolated by manual or automated ficoll isolation and seeded in T175 flasks. After a P0 expansion of 14 days, cells were harvested and re-loaded in new flasks for P1 expansion. One week later, cells were harvested and re-loaded according to the same scheme for P2 expansion before a final harvest around day 28. Quality controls were performed at different stages of the process