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. 2016 May 20;14:145. doi: 10.1186/s12967-016-0892-y

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — MSC immunosuppressive properties. Inhibition of PBMC proliferation by third party MSC: PBMC (100,000 or 50,000) were stimulated (S-PBMC) with anti-αCD3/CD28 microbeads during 4 days with or without irradiated (25 Gy) MSC (10/1 or 5/1 PBMC/MSC ratios) added at the beginning of the culture. Proliferation was assessed by analysis of the cell cycle by flow cytometry (N = 28). Result are expressed as the percentage of cells present in S + G2 M phases (a) and as the percentage of inhibition compared to the stimulated PBMC condition alone (b) for the 10/1 PBMC/MSC ratio